sisti: Real-Time PCR Data Sets by Sisti et al. (2010)
This data package contains four datasets of quantitative PCR (qPCR)
amplification curves that were used as supplementary data in the research
article by Sisti et al. (2010), <doi:10.1186/1471-2105-11-186>.
The primary dataset comprises a ten-fold dilution series spanning
copy numbers from 3.14 × 10^7 to 3.14 × 10^2, with twelve replicates per
concentration. These samples are based on a pGEM-T Promega plasmid containing
a 104 bp fragment of the mitochondrial gene NADH dehydrogenase 1 (MT-ND1),
amplified using the ND1/ND2 primer pair. The remaining three datasets contain
qPCR results in the presence of specific PCR inhibitors: tannic acid,
immunoglobulin G (IgG), and quercetin, respectively, to assess their effects
on the amplification process. These datasets are useful for researchers
interested in PCR kinetics. The original raw data file is available as
Additional File 1:
<https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-11-186/MediaObjects/12859_2009_3643_MOESM1_ESM.XLS>.
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[aut, cre],
Pattern Institute [cph, fnd]